The Thermo Scientific OGeneRuler 1 kb DNA Ladder ReadyToUse 25010000 delivers precise, pre-mixed sizing for demanding molecular workflows. This ready-to-use ladder covers a broad range and simplifies loading so researchers can focus on interpreting clear band patterns.
Engineered for consistent performance, this ladder supports fast verification and confident size estimation across agarose and native gels. Below is a detailed snapshot of its intended use, format, and performance features.
| Product Name | Thermo Scientific OGeneRuler 1 kb DNA Ladder |
|---|---|
| Catalog Number | 25010000 |
| Size Range | 100 bp to 10,000 bp |
| Format | ReadyToUse, pre-loaded with tracking dye |
| Typical Storage | Stable at 4°C; avoid repeated freeze-thaw |
Precise Sizing Across a Wide Range
Each band in the Thermo Scientific OGeneRuler 1 kb ladder corresponds to a known fragment length, enabling accurate estimation of target amplicons. The distinct spacing between bands supports confident size inference even in complex samples.
Performance Highlights
Designed for high consistency, this ladder minimizes variability between runs. Sharp, well-resolved bands allow reliable detection in both agarose and pulsed-field formats under standard imaging conditions.
Optimized Workflow for Researchers
The ready-to-use format eliminates tube changes and pipetting steps, accelerating setup and reducing potential for error. Laboratories performing routine diagnostics or screening benefit from faster turnaround without sacrificing resolution.
Streamlined Handling
Supplied in a stable, ready-to-load format, this ladder integrates seamlessly into standard protocols. Clear labeling and a robust formulation support seamless integration into existing workflows.
Analytical Performance and Applications
Extensive testing shows reliable band intensity and position across a broad concentration range. This makes the ladder suitable for verifying restriction digests, confirming PCR amplicons, and validating cloning steps.
Compatibility Considerations
Formulated to remain stable in common running buffers, the ladder performs well in both agarose and pulsed-field gel systems. It is compatible with standard UV and fluorescence imaging setups without requiring specialized detection methods.
Specifications at a Glance
Key performance characteristics and format details are summarized for rapid reference. Use this table to confirm that the ladder matches your gel system and expected size targets.
| Attribute | Specification | Unit | Typical Value |
|---|---|---|---|
| Size Range | Smallest to Largest Fragment | bp | 100 to 10,000 |
| Concentration | DNA Content | ng/μL | ~50 |
| Volume per Load | Recommended Loading Volume | μL | 1–5 |
| Storage Conditions | Short-Term and Long-Term Stability | °C | 4°C; avoid freeze-thaw |
Frequently Asked Questions
Can I use this ladder for pulsed-field gel electrophoresis?
Yes, the broad range and consistent band pattern make it suitable for pulsed-field applications when paired with appropriate running conditions.
How should I store the Thermo Scientific OGeneRuler 1 kb ladder to maintain stability?
Store at 4°C and avoid repeated freeze-thaw cycles to preserve band integrity and performance over time.
Will this ladder work with SYBR Safe or other nucleic acid stains?
Yes, the formulation is compatible with SYBR Safe and similar stains, producing sharp bands under standard UV or fluorescence imaging.
What volume of ladder should I load for clear band visualization?
Loading 1–5 μL typically provides sufficient signal for clear visualization, depending on gel concentration and imaging system sensitivity.
Recommended Practices with OGeneRuler 1 kb Ladder
Adopting consistent handling and storage routines helps maintain reproducibility and band clarity across experiments.
- Always avoid repeated freeze-thaw cycles to preserve ladder integrity.
- Load 1–5 μL depending on gel concentration and imaging sensitivity.
- Verify band positions periodically against known standards.
- Store at 4°C in a dedicated location to prevent cross-contamination.
- Use compatible staining methods for optimal visualization under UV or fluorescence.
